Proteins and proteomics : a laboratory manual
作者： Richard J Simpson , Richard J Simpson
DOI:
关键词: Biology 、 Gel electrophoresis 、 Phosphorylation sites 、 Sequence analysis 、 A protein 、 Peptide mapping 、 Proteomics 、 Polyacrylamide gel electrophoresis 、 Chromatography 、 Biochemistry
摘要: Chapter 1. Introduction to Proteomics 2. One-dimensional Polyacrylamide Gel Electrophoresis 3. Preparing Cellular and Subcellular Extracts 4. Preparative Two-dimensional with Immobilized pH Gradients 5. Reversed-phase High-performance Liquid Chromatography 6. Amino- Carboxy-terminal Sequence Analysis 7. Peptide Mapping of Gel-resolved Proteins 8. The Use Mass Spectrometry in 9. Proteomic Methods for Phosphorylation Site 10. Characterization Protein Complexes 11. Making Sense Proteomics: Using Bioinformatics Discover a Protein's Structure, Functions, Interactions
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