Observations on Pine Embryos Grown In vitro

摘要: 1. Mature embryos of Pinus lambertiana Dougl. were excised aseptically from seeds stratified at 5⚬ C. for 6 weeks and grown in light on the surface a medium containing inorganic nutrients, 2% sucrose, 0.8% agar. Normal, healthy seedlings resulted. Growth sequences paralleled those vivo germinations, but initial growth responses more rapid culture. The addition various factors produced no added benefits. 2. terminal bud was normal even when cotyledons not contact with agar medium. This contrasts results previous study Ginkgo shows that haustorial function can be by-passed Submerging entire embryo or hypocotylary end beneath generally resulted abnormal, tumorous development hypocotyl root. 3. After 3 culture, root elongation ceased, shoot consisted slow of...