PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.
作者： Aining Li , Yonglin Wang , Kai Tao , Suomeng Dong , Qian Huang
关键词: Spore germination 、 Pleckstrin homology domain 、 Phytophthora sojae 、 Hyphal growth 、 Germ tube 、 Zoospore 、 Wild type 、 Biology 、 Oospore 、 Microbiology
摘要: Mitogen-activated protein kinase (MAPK) pathways are universal and evolutionarily conserved signal transduction modules in all eukaryotic cells. In this study, PsSAK1, which encodes a stress-activated MAPK of Phytophthora sojae, was identified. PsSAK1 is highly oomycetes, it represents novel group due to its pleckstrin homology domain. Reverse-transcription polymerase chain reaction analysis showed that expression upregulated zoospores cysts during early infection. addition, induced by osmotic oxidative stress mediated NaCl H(2)O(2), respectively. To elucidate the function, silenced using stable transformation P. sojae. The silencing did not impair hyphal growth, sporulation, or oospore production but severely hindered zoospore development, strains quicker encystment lower germination ratio than wild type. PsSAK1-silenced mutants produced much longer germ tubes could colonize either wounded unwounded soybean leaves. Our results indicate an important regulator development pathogenicity
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