Virus-ligand interactions: identification and characterization of ligand binding by NMR spectroscopy.

摘要: We demonstrate the detection and characterization of ligand binding to viruses via NMR. To illustrate methodology, interaction an antiviral compound with human rhinovirus serotype 2 (HRV2) was investigated. Specific a capsid-binding inhibitor native HRV2 monitored utilizing saturation transfer difference (STD) STD NMR experiments at atomic resolution allowed those regions that are involved in virus be determined. The approach allows for (i) fast robust assessment binding, (ii) determination epitope without necessity crystallize virus−ligand complexes, (iii) reuse subsequent assays. This methodology enables one easily identify drugs, peptides, receptor or antibody fragments viral capsid.