The Development of a Th1-Type Response and Resistance toLeishmania majorInfection in the Absence of CD40-CD40L Costimulation

摘要: CD40-CD40L interactions have been shown to be essential for the production of IL-12 and IFN-γ control L. major infection. In contrast, C57BL/6 mice deficient in CD28 develop a dominant Th1-type response heal this study, we investigate effects deficiency both CD40L molecules on immune course We compared infection genetically lacking (CD40L−/−), (CD28−/−), or (CD40L−/−CD28−/−), mice, all resistant background. Although CD40L−/− failed infection, CD28−/− CD40L−/−CD28−/− as well spontaneously resolved their infections. Healing had reduced numbers lesion parasites with nonhealing mice. At wk 9 detected similar levels IL-4, IFN-γ, IL-12p40, IL-12Rβ2 mRNA draining lymph nodes healing C57BL/6, CD28−/−, whereas increased IL-4 but IL-12Rβ2. separate experiment, blocking pathway using Ab led an exacerbation little no effect Together, these results demonstrate that absence costimulation, interaction is not required development protective response. The expression IL-12Rβ2, further suggests presence additional stimulus capable regulating its receptors interactions.