IL-12 Receptor (IL-12R) Expression and Accumulation of IL-12Rβ1 and IL-12Rβ2 mRNAs in CD4+ T Cells by Costimulation with B7-2 Molecules
作者： Shinobu Imajoh-Ohmi , Hideo Nariuchi , Hidehiro Yamane , Osamu Igarashi
DOI:
关键词: Cytokine 、 Il 12rβ2 、 Stimulation 、 Molecular biology 、 Clone (cell biology) 、 Biology 、 Chinese hamster ovary cell 、 Cell biology 、 Interleukin 12 、 Messenger RNA 、 Receptor
摘要: IL-12 is a crucial cytokine for the determination of Th1/Th2 balance. It important, therefore, to elucidate mechanisms IL-12R expression on Th cells. In this report, we present evidence show that B7-2 costimulation plays pivotal role in A Th1 clone expressed low density resting condition, was enhanced by stimulation with specific Ag splenic adherent cells and enhancement inhibited anti-B7-2 or CTLA-4-Ig. When stimulated anti-CD3 plus B7-2-transfected Chinese hamster ovary (CHO) cells, strongly IL-12R, although itself only weakly expression. We obtained results were similar those CD4+CD45RBlow memory T CD4+CD44low naive B7-2-CHO found play more important The accumulation both IL-12Rβ1 -β2 chain mRNAs detected when they costimulated B7-2-CHO, but β2 mRNA not upon alone. On other hand, clones amounts these without any stimulation, For maximum mRNAs, however, also required B7-2-CHO.
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