COVID-19: CRISPR/Cas-like System of nsp3 Promotes the Mutant Recombination and Drug Resistance (preprint)

摘要: Patients with novel coronavirus pneumonia usually suffer from bacterial and fungal infections, the drug resistance problem caused by pandemic is becoming more serious Simultaneously, SARS-COV-2 virus has a rapid mutation phenomenon, somegene coding regions recombination may be related to of Therefore, studying relationship between co-infection bacteria fungi evolution important guiding significance for preventing We found that virus's nsp3 protein had CRISPR/Cas 9 (II-B)-like function searching conserved domains The system could target edit negative-strand RNA speculated crRNA (CRISPR RNA) produced Pseudomonas aeruginosa carried genetic information bacteriophages Pseudomonas, including After phage lysed was released attached spores, then invaded patient's cells along spores or hyphae synthesized assembled 4Fe-4S, iron-containing molecules bound cas4 domain, in mitochondria phagocytes iron came hemoglobin attacked phagocytic cytoplasm It targeted SARS-COV-2, inserting domain gene fragments into through editing splicing Since Cas no codon checking function, cutting would destroy protein-coding original region, causing genome If phage, have similar Because growing COVID-19 patients, we should pay attention Avoid CRISPR/Cas-like cause increased