The Dissociation of Rabbit Reticulocyte Ribosomes with EDTA and the Location of Messenger Ribonucleic Acid
作者： R. D. Nolan , H. R. V. Arnstein
DOI: 10.1111/J.1432-1033.1969.TB00629.X
关键词: Sedimentation coefficient 、 Protein biosynthesis 、 Density gradient 、 Sucrose 、 Phenylalanine 、 Reticulocyte 、 Ribosome 、 Chemistry 、 Centrifugation 、 Chromatography 、 Biochemistry
摘要: Rabbit reticulocyte ribosomes were incubated with polyuridylic acid at 37° for 1 h under conditions suitable protein synthesis. Ribosomes containing bound isolated and dissociated into subparticles by treatment EDTA. The separated zone centrifugation in a sucrose density gradient the distribution of was examined measuring ability fractions to stimulate incorporation [14C] phenylalanine cell-free system. It observed that peak activity corresponded region subparticle having sedimentation coefficient about 50 S. When used initial binding reaction, subsequent analysis produced EDTA confirmed presence on or close large ribosomal subparticle.
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