Separatecis-transPathways Post-transcriptionally Regulate Murine CD154 (CD40 Ligand) Expression
作者： B. JoNell Hamilton , Xiao-Wei Wang , Jane Collins , Donald Bloch , Alan Bergeron
关键词: Messenger RNA 、 Three prime untranslated region 、 Response element 、 Function (biology) 、 CD154 、 Heterogeneous-Nuclear Ribonucleoprotein L 、 Regulation of gene expression 、 Molecular biology 、 Biology 、 Translation (biology) 、 Cell biology 、 Biochemistry
摘要: We report a role for CA repeats in the 3′-untranslated region (3′-UTR) regulating CD154 expression. Human is encoded by an unstable mRNA; this instability conferred cis portion of its 3′-UTR that includes polypyrimidine-rich and dinucleotide repeat. demonstrate similar activity with murine 3′-UTR. This element mapped solely to conserved 100-base CU-rich alone, which we call response element. Surprisingly, dinucleotide-rich also regulated reporter expression but at level translation. was associated poly(A) tail shortening heterogeneous nuclear ribonucleoprotein L levels. conclude contains dual cis-acting elements, one defines novel function exonic repeats. These findings suggest mechanism association CA-rich polymorphisms overexpression subsequent risk autoimmune disease.
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