[6] Improved phosphotriester method for the synthesis of gene fragments
作者： S.A. Narang , Hansen M. Hsiung , Roland Brousseau
DOI: 10.1016/0076-6879(79)68008-4
关键词: Phosphodiester bond 、 Silica gel 、 Organic chemistry 、 Oligonucleotide 、 Chemistry 、 Guanine 、 Solvent 、 Chromatography 、 Yield (chemistry)
摘要: Publisher Summary This chapter discusses an experimental study focusing on the improved phosphotriester method for synthesis of gene fragments. During studies with a modified triester approach, it was observed that pure, fully protected product difficult to separate quantitatively by conventional silica gel chromatography from crude reaction mixture, especially components containing several guanine bases. It found thin-layer (TLC) silanized (RP-2) and KC 18 (RP-18) plates in acetone–water solvent gave excellent separation trityl hydroxyl groups also those differing sizes. The polar component (containing 3′ phosphodiester) generally moved front, group) slowest. mobility 5′-hydroxyl group between nonpolar compounds. purification GGCA could not be achieved as determined after complete deblocking TLC polyethyleneimine (PEI), whereas reverse-phase pure analyzed PEI plate. These results indicated oligonucleotide yield at each step synthesis.
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