Nonaqueous solutions of DNA. Reversible and irreversible denaturation in methanol.
作者： E.Peter Geiduschek , Theodore T. Herskovits
DOI: 10.1016/0003-9861(61)90116-3
关键词: Methanol 、 Ionic strength 、 Denaturation (biochemistry) 、 Crystallography 、 Chemistry 、 Aqueous solution 、 Photochemistry 、 Macromolecule 、 Irreversible denaturation 、 Protein secondary structure 、 DNA
摘要: Abstract Deoxyribonucleic acid (DNA) undergoes denaturation in methanol-water mixtures. The region of solvent composition which this transition occurs depends on the ionic strength and temperature but is little influenced by average nucleotide DNA. As judged both macromolecular optical criteria, disruption secondary structure almost complete, yet methanol rapidly readily reversible water. structural basis reversibility differs from that controlling partial aqueous solution. An irreversible thermal has also been found to occur methanol-rich solvents. products may be reconverted native DNA
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sci-hub.se 参考文章(23)
John T. Edsall, Alexander Rich, Martin Goldstein, An Instrument for the Study of Double Refraction of Flow at Low and Intermediate Velocity Gradients Review of Scientific Instruments. ,vol. 23, pp. 695- 701 ,(1952) , 10.1063/1.1746142
John G. Kirkwood, Richard J. Goldberg, Light Scattering Arising from Composition Fluctuations in Multi‐Component Systems The Journal of Chemical Physics. ,vol. 18, pp. 54- 57 ,(1950) , 10.1063/1.1747456
R. A. COX, U. Z. LITTAUER, Secondary structure of ribonucleic acid in solution. Nature. ,vol. 184, pp. 818- 819 ,(1959) , 10.1038/184818A0
J.H. Coates, D.O. Jordan, Deoxypentose nucleic acids XV. The sedimentation of calf thymus deoxyribonucleic acid in 95% ethanol Biochimica et Biophysica Acta. ,vol. 43, pp. 214- 222 ,(1960) , 10.1016/0006-3002(60)90432-7
Sidney Katz, The Reversible Reaction of Sodium Thymonucleate and Mercuric Chloride Journal of the American Chemical Society. ,vol. 74, pp. 2238- 2245 ,(1952) , 10.1021/JA01129A023
W. H. Stockmayer, Light Scattering in Multi‐Component Systems The Journal of Chemical Physics. ,vol. 18, pp. 58- 61 ,(1950) , 10.1063/1.1747457
Vincent L. Stevens, Edward L. Duggan, Deformation of Deoxyribonucleate. II. Precipitation of Heat-deformed DNA with Millimolar Lead Ion1 Journal of the American Chemical Society. ,vol. 79, pp. 5703- 5706 ,(1957) , 10.1021/JA01578A031
U.Z. Littauer, H. Eisenberg, Ribonucleic acid from Escherichia coli preparation, characterization and physical properties Biochimica et Biophysica Acta. ,vol. 32, pp. 320- 337 ,(1959) , 10.1016/0006-3002(59)90604-3
Lyle G. Bunville, E.Peter Geiduschek, DNA composition and stability to acid denaturation Biochemical and Biophysical Research Communications. ,vol. 2, pp. 287- 292 ,(1960) , 10.1016/0006-291X(60)90186-8
A.S. Jones, The isolation of bacterial nucleic acids using cetyltrimethylammonium bromide (Cetavlon) Biochimica et Biophysica Acta. ,vol. 10, pp. 607- 612 ,(1953) , 10.1016/0006-3002(53)90304-7