Catalytic single-chain polymeric nanoparticles at work: from ensemble towards single-particle kinetics

摘要: Folding a single polymer chain around catalytically active sites to construct catalytic polymeric nanoparticles (SCPNs) is novel approach mimic the activity and selectivity of enzymes. In order relate efficiency SCPNs their three-dimensional structure, better understanding at an individual level, rather than ensemble highly desirable. this work, we present design preparation family fluorogenic substrates, characterization level as well our progress towards analyzing with single-molecule fluorescence microscopy (SMFM). Firstly, organocopper-based together rhodamine-based substrates were designed synthesized. The catalyze carbamate cleavage reaction mono-protected rhodamines, dimethylpropargyloxycarbonyl protecting group being cleaved most efficiently. A systematic study focusing on conditions during catalysis revealed that ligand acceleration effect accumulation in significantly promote performance. Secondly, streptavidin–biotin based strategy was developed immobilize surface glass coverslips. Fluorescence correlation spectroscopy experiments confirmed remained after immobilization. Finally, single-SCPN measurements performed. results qualitatively indicated fluorescent product molecules formed result could be detected. So far, no evidence for strongly different behaviors has been observed when comparing SCPNs.