Gene expression in hair follicle dermal papilla cells after treatment with stanozolol

摘要: Doping with anabolic agents is a topic in sports where strength crucial, e.g. sprinting, weight lifting and many more. Testosterone its functional analogs are the drugs of choice taken as pills, creams, tape or injections to increase muscle mass body performance, reduce fat. Stanozolol (17β-hydroxy-17α-methyl-5α-androst- 2-eno[3,2c]pyrazol) testosterone analogue same effect like but ring structure makes it possible take orally. Therefore, stanozolol one most frequently used steroids. Common verification methods for exist, identifying chemicals tissues, hair blood samples. The idea this feasibility study was search specific gene expression regulations induced by identify influence synthetically hormone on different metabolic pathways. Finding biomarkers could be supportive existing an additional proof illegal drug abuse. In two separate cell cultures, human HFDPC (hair follicle dermal papilla cells) from female male donor were treated stanozolol. culture treatment concentrations 0 nM (control), 1 nM, 10 100 chosen. Cells h, 6 24 h 48 after stimulation totalRNA extracted. Learning results pilot experiment, 72 h. Using quantitative real-time RT-PCR characteristics target genes analysed. Totally 13 selected according their functionality screening actual literature composed groups: factors apoptosis regulation Fas Ligand (FasL), receptor (FasR), Caspase 8 Bcl-2. Androgen (AR) both estrogen receptors (ERα, ERβ) summarized steroid group. growth factor group included insulin (IGF1R) (GHR). Fibroblast 2 (FGF2) keratinocyte (FGF7) cycle 5α-Steroidreductases (SRD5A1, SRD5A2) represented enzyme Three reference relative quantification: ubiquitin (UBQ), glycerinaldehyde-3-phsophate-dehydrogenase (GAPDH), β-actin (ACTB). AR, FasR, FGF2 showed significant within time, expressions over time analysed 8. FasR SRD5A2 significantly regulated time. first biomarker pattern identified providing rationality investigate modified, pathways whole follicle.