Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers

摘要: Abstract With this feasibility study a first step towards new monitoring system for hormonal treatments was done. Screening of regulation and function anabolic sex steroids via modified gene expression mRNA in various tissues could be approach to trace with unknown drugs or newly combined cocktails. In the study, uterus, liver muscle tissue from 24 cycling heifers were taken after animals treated either Melengestrol Acetate (MGA), Finaplix-H® (200 mg Trenbolone Acetate) Ralgro® (36 mg Zeranol) 56 days. every treatment group always two given 1-fold, 3-fold 10-fold doses standard preparation, control without any consisted animals. The different profiles investigated candidate approach. Totally 57 genes selected according their functionality by screening actual literature composed functional groups: angiogenesis, apoptosis, cell cycle, endocrine factors, energy metabolism, inflammatory function, oncogenes, protein metabolism transcription factors. Gene measured using quantitative real-time RT-PCR (qRT-PCR) technology. From tested liver, 17 showed significant regulation. Eight influenced MGA, 9 Finaplix-H®, 4 Ralgro®. For 19 result that neck 11 regulated hind limb 8 genes. 5 affected 6 3 Only 2 MGA muscle. uterus 29 target 13 significantly steroids. Under eight four highest changes under observed uterus. analyzed regulations but further studies, testing animal husbandry conditions will needed identify meaningful patterns tissues. investigation possible expression, preparatory work development an pattern drug made.