A rapid LC–MS/MS method for quantitative profiling of fatty acids, sterols, glycerolipids, glycerophospholipids and sphingolipids in grapes

摘要: The abundance of lipids in plants is influenced by genotype and phenotype. Despite being a very important class plant metabolites, knowledge grape still limited to date, with the exception those located seeds. Few investigations have shown that their profile depends on maturity, variety location berry. Recent advances liquid chromatography coupled mass spectrometry paved way for faster analysis minimal sample preparation. Here we describe validation method extraction, identification quantification different classes lipids: fatty acids, sterols, glycerolipids, glycerophospholipids sphingolipids using chromatographic electrospray ionization tandem (LC-ESI-MS/MS). was validated 33 lipids, linearity range (R(2)=0.95-1.00), LOQ (0.003-14.88 ng mL(-1)) intraday interday repeatability evaluated each lipid. lipid profiling developed successfully applied 18 samples (10 red 8 white varieties) from 4 genetic groups: Vitis vinifera, non-vinifera, Muscat hybrid; were identified quantified. This method, which can be easily expanded include further compounds other tissues, starting point an essential goal better understanding role physiology.