Comparison of blood polymerase chain reaction and enzyme-linked immunosorbent assay for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep.
作者： Ramon A. Juste , Joseba M. Garrido , Marivi Geijo , Natalia Elguezabal , Gorka Aduriz
DOI: 10.1177/104063870501700409
关键词: Enzyme 、 Flock 、 Mycobacterium avium subsp. paratuberculosis 、 Fisher's exact test 、 Paratuberculosis 、 Polymerase chain reaction 、 Kappa 、 Biology 、 Animal science
摘要: A study was carried out to compare the performance of enzyme-linked immunosorbent assay (ELISA) and blood polymerase chain reaction (PCR) for diagnosis paratuberculosis in cattle sheep. For cattle, a set 278 samples from 1 paratuberculosis-affected Friesian farm used; it included 80 ELISA-positive 198 ELISA-negative an age-matched group. Ninety-four were heifers 184 2–5-year-old cows. The overall analysis showed clear association (Fisher exact test [FET] P = 0.0049) but weak negative agreement (45.3%, kappa −0.1665 ± 0.0994) between 2 tests. It reflected moderate among (87.7%, 0.4471 0.2435) disagreement cows (62.7%, −0.3670 0.1057). sheep, 496 53 Latxa dairy flocks 180 dam/offspring pairs. tests on ovine strong (FET, 0.0005), whereas l...
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