Direct bacterial loop-mediated isothermal amplification detection on the pathogenic features of the nosocomial pathogen – Methicillin resistant Staphylococcus aureus strains with respiratory origins

摘要: Loop-mediated isothermal amplification based detection assays using bacterial culture or colony for direct of methicillin resistant Staphylococcus aureus(MRSA) had been developed and evaluated, followed by its extensive application on a large scale clinical MRSA isolated from respiratory origins, including nasal swabs sputums. Six primers, outer inner primers loop were specifically designed recognizing eight distinct sequences four targets: 16SrRNA, femA, mecA orfX. Twenty-seven reference strains used to develop, evaluate optimize this assay. Then, total 532 isolates employed each detected targets. And the results determined through both visual observation color change naked eye electrophoresis. The specific primer confirmed, optimal was obtained under 65 °C 40 min. limit detections (LOD) bacteria LAMP be 104 CFU/ml 16S rRNA, as well orfX 105 mecA, respectively. established novel may provide new strategies rapid foodborne pathogens.