In vitro skin toxicity of CuO and ZnO nanoparticles: Application in the safety assessment of antimicrobial coated textiles

摘要: Abstract In the context of nosocomial infections, there is an urgent need to develop efficient nanomaterials (NMs) with antibacterial properties for prevention infection diseases. Metal oxide nanoparticles (MeO-NPs) are promising candidates development new textiles. However, direct exposure MeO-NPs and MeO-coated NMs through skin contact could constitute a severe hazard human health. this work, toxicity copper zinc (CuO, ZnO) NPs antimicrobial-coated textiles was assessed on in vitro reconstructed 3D model epidermis. Thus, extracts from were tested EpiDerm™ according OECD TG 431 (Corrosion Test) 439 (Irritation Test), respectively. Skin surface fluids composition crucial aspect be considered that have encounter tissue. So, irritation test, coated extracted artificial sweat solutions at pH 4.7 6.5. tissue viability, pro-inflammatory interleukin-8 secretion morphological alteration intermediate actin filaments keratinocytes evaluated after 18 h CuO- ZnO-coated Analysis two pH conditions indicated released ions not involved promoting adverse effects Since Cu2+ Zn2+ known penetrate epidermis, Balb/3 T3 cells used as dermis. Fibroblasts viability investigated trans-epidermis permeated ions, collected basal supernatants, extracts, representative interaction dermis by wounded skin. From our data we can conclude that: 1) key parameter stability NPs-coated textiles; 2) MeO deeply affect epidermal underlying dermal upon trans-epidermal permeation; 3) barrier integrity fundamental prerequisite should taken into account during assessment safety exposure.