The influence of adjuvants on the immunological response of the chicken: II. Effects of Freund's complete adjuvant on later antibody production after a single injection of immunogen

摘要: A study has been made in the chicken of late phase (after 21st day) immunological response to a single intramuscular injection soluble protein antigen, human serum albumin (HSA), incorporated water-in-oil emulsion with or without heat-killed Mycobacterium tuberculosis. Birds injected showed, after fall from initial primary peak (8–12th day), second rise antibody production beginning at day. Control birds HSA 0.15 M NaCl failed show this rise. The these experiments fell between 42nd and 59th days. results one experiment showed that Freund's complete adjuvant average level was 100-fold higher than first level. However, reached much those simple emulsion. The avidity anti-HSA present by emulsions compared produced during (10th day). sera obtained given added M. tuberculosis greater phase. The anatomical site investigated. findings suggest that, whereas is spleen probably other lymphoid tissues, may be granuloma emulsion.