RhoA and Cytoskeletal Disruption Mediate Reduced Osteoblastogenesis and Enhanced Adipogenesis of Human Mesenchymal Stem Cells in Modeled Microgravity
作者： Valerie E Meyers , Majd Zayzafoon , Joanne T Douglas , Jay M McDonald
DOI: 10.1359/JBMR.050611
关键词: Mesenchymal stem cell 、 Cellular differentiation 、 Biology 、 Cytoskeleton 、 Cell biology 、 Adipogenesis 、 Stress fiber 、 Osteoblast 、 RHOA 、 Stem cell 、 Biochemistry
摘要: Spaceflight, aging, and disuse lead to reduced BMD. This study shows that overexpression of constitutively active RhoA restores actin cytoskeletal arrangement, enhances the osteoblastic phenotype, suppresses adipocytic phenotype human mesenchymal stem cells cultured in modeled microgravity. Introduction: Reduced BMD during spaceflight is partly caused by bone formation. However, mechanisms responsible for this loss remain unclear. We have previously shown osteoblastogenesis enhanced adipogenesis (hMSCs) microgravity (MMG). The small GTPase, RhoA, regulates stress fiber formation has been implicated lineage commitment hMSCs. examined effects MMG on organization activity ability reverse these effects.
sci-hub.se 参考文章(47)
Millie Hughes-Fulford, Physiological effects of microgravity on osteoblast morphology and cell biology. Advances in Space Biology and Medicine. ,vol. 8, pp. 129- 157 ,(2002) , 10.1016/S1569-2574(02)08017-6
T. G. Hammond, J. M. Hammond, Optimized suspension culture: the rotating-wall vessel American Journal of Physiology-renal Physiology. ,vol. 281, ,(2001) , 10.1152/AJPRENAL.2001.281.1.F12
Anne Caillot-Augusseau, Marie-Héléne Lafage-Proust, Claude Soler, Josiane Pernod, Francis Dubois, Christian Alexandre, None, Bone formation and resorption biological markers in cosmonauts during and after a 180-day space flight (Euromir 95) Clinical Chemistry. ,vol. 44, pp. 578- 585 ,(1998) , 10.1093/CLINCHEM/44.3.578
T. J. Goodwin, T. L. Prewett, David A. Wolf, G. F. Spaulding, Reduced shear stress: A major component in the ability of mammalian tissues to form three‐dimensional assemblies in simulated microgravity Journal of Cellular Biochemistry. ,vol. 51, pp. 301- 311 ,(1993) , 10.1002/JCB.240510309
Lora Giangregorio, Cameron J. Blimkie, Skeletal adaptations to alterations in weight-bearing activity: a comparison of models of disuse osteoporosis. Sports Medicine. ,vol. 32, pp. 459- 476 ,(2002) , 10.2165/00007256-200232070-00005
L.B. Buravkova, Yu.A. Romanov, The role of cytoskeleton in cell changes under condition of simulated microgravity Acta Astronautica. ,vol. 48, pp. 647- 650 ,(2001) , 10.1016/S0094-5765(01)00023-6
Yasuhiro Takeuchi, Miyuki Suzawa, Tomoko Kikuchi, Eisuke Nishida, Toshiro Fujita, Toshio Matsumoto, Differentiation and Transforming Growth Factor-β Receptor Down-regulation by Collagen-α2β1 Integrin Interaction Is Mediated by Focal Adhesion Kinase and Its Downstream Signals in Murine Osteoblastic Cells Journal of Biological Chemistry. ,vol. 272, pp. 29309- 29316 ,(1997) , 10.1074/JBC.272.46.29309
Laurence Vico, Philippe Collet, Alain Guignandon, Marie-Hélène Lafage-Proust, Thierry Thomas, Mohamed Rehailia, Christian Alexandre, None, Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts The Lancet. ,vol. 355, pp. 1607- 1611 ,(2000) , 10.1016/S0140-6736(00)02217-0
Dympna Harmey, Gudrun Stenbeck, Catherine D Nobes, Alistair J Lax, Agamemnon E Grigoriadis, Regulation of osteoblast differentiation by Pasteurella multocida toxin (PMT): a role for Rho GTPase in bone formation. Journal of Bone and Mineral Research. ,vol. 19, pp. 661- 670 ,(2004) , 10.1359/JBMR.040105
Raffaella Sordella, Wei Jiang, Guang-Chao Chen, Marcello Curto, Jeffrey Settleman, Modulation of Rho GTPase Signaling Regulates a Switch between Adipogenesis and Myogenesis Cell. ,vol. 113, pp. 147- 158 ,(2003) , 10.1016/S0092-8674(03)00271-X