Phytophthora Effectors Modulate Genome-wide Alternative Splicing of Host mRNAs to Reprogram Plant Immunity.

摘要: Abstract Alternative splicing (AS) of pre-mRNAs increases transcriptome and proteome diversity, regulates gene expression through multiple mechanisms, plays important roles in plant development stress responses. However, the prevalence genome-wide AS changes during infection mechanisms by which pathogens modulate remain poorly understood. Here, we examined global tomato leaves infected with Phytophthora infestans, infamous Irish famine pathogen. We show that more than 2000 genes exhibiting significant are not differentially expressed, indicating is a distinct layer reprogramming plant–pathogen interactions. Furthermore, our results P. infestans subverts host immunity repressing positive regulators promoting susceptibility factors. To study underlying mechanism, established luminescence-based reporter system Nicotiana benthamiana to screen pathogen effectors modulating AS. identified nine regulatory (SREs) from 87 P. infestans effectors. Further studies revealed SRE3 physically binds U1-70K manipulate machinery subsequently modulates AS-mediated immunity. Our only unveils but also establishes novel screening tool identify SREs wide range pathogens, providing opportunities understand subvert