Phylogenetic comparison of 5' splice site determination in central spliceosomal proteins of the U1-70K gene family, in response to developmental cues and stress conditions.

摘要: Intron-containing genes have the ability to generate multiple transcript isoforms by splicing, thereby greatly expanding eukaryotic transcriptome and proteome. In cells, precursor mRNA (pre-mRNA) splicing is performed a mega-macromolecular complex defined as spliceosome. Among its components, U1 small nuclear ribonucleoprotein (U1 snRNP) smallest subcomplex involved in early spliceosome assembly 5'-splice site recognition. Its central component, named U1-70K, has been extensively characterized animals yeast. Very few investigations on U1-70K conducted plants, however. To this end, we comprehensive study systematically identify 115 from 67 plant species, ranging algae angiosperms. Phylogenetic analysis suggested that expansion of gene family was likely driven whole-genome duplications. Subsequent comparisons structures, protein domains, promoter regions conserved patterns indicated U1-70Ks are preserve their molecular function across lineages play an important functional role response environmental stresses. Furthermore, genetic using T-DNA insertion mutants Arabidopsis may be osmotic stress. Our results provide general overview Viridiplantae will act reference source for future mechanistic studies snRNP-specific factor.