Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?
作者： Inna Serganova , Ronald G. Blasberg
DOI: 10.2967/JNUMED.118.220004
关键词: Cell signaling 、 Biology 、 Surrogate measure 、 Computational biology 、 Gene 、 Cell tracking 、 Molecular imaging 、 Translation (biology) 、 Reporter gene 、 Transcription factor
摘要: The first reporter systems were developed in the early 1980s and based on measuring activity of an enzyme-as a surrogate measure promoter-driven transcriptional activity-which is now known as gene system. initial objective application techniques was to analyze specific promoter (namely, expression that under regulation linked gene). This system allows visualization with great sensitivity. In general, there are 2 classes systems: constitutively expressed (always-on) constructs used for cell tracking, inducible sensitive endogenous signaling molecules transcription factors characterize tissues, tumors, or pathways.This review traces development different systems, using fluorescent bioluminescent proteins well radionuclide-based systems. focus this review. question at end whether "promise" imaging has been realized. What required moving forward what successful translation clinical applications?
sci-hub.se 参考文章(225)
H.F. Gallardo, C. Tan, D. Ory, M. Sadelain, Recombinant Retroviruses Pseudotyped With the Vesicular Stomatitis Virus G Glycoprotein Mediate Both Stable Gene Transfer and Pseudotransduction in Human Peripheral Blood Lymphocytes Blood. ,vol. 90, pp. 952- 957 ,(1997) , 10.1182/BLOOD.V90.3.952
Grant R. MacGregor, Garry P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg, Use of Escherichiu coli (E. coli) lacZ (β-Galactosidase) as a Reporter Gene. Methods of Molecular Biology. ,vol. 7, pp. 217- 235 ,(1991) , 10.1385/0-89603-178-0:217
Greetje Vande Velde, Uwe Himmelreich, Michal Neeman, Reporter gene approaches for mapping cell fate decisions by MRI: promises and pitfalls. Contrast Media & Molecular Imaging. ,vol. 8, pp. 424- 431 ,(2013) , 10.1002/CMMI.1590
Angelique Y. Louie, Martina M. Hüber, Eric T. Ahrens, Ute Rothbächer, Rex Moats, Russell E. Jacobs, Scott E. Fraser, Thomas J. Meade, In vivo visualization of gene expression using magnetic resonance imaging Nature Biotechnology. ,vol. 18, pp. 321- 325 ,(2000) , 10.1038/73780
P E Jacob, C V Hall, G M Ringold, F Lee, Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells. Journal of molecular and applied genetics. ,vol. 2, pp. 101- 109 ,(1983)
Laura Mezzanotte, Markus Aswendt, Annette Tennstaedt, Rob Hoeben, Mathias Hoehn, Clemens Löwik, Evaluating reporter genes of different luciferases for optimized in vivo bioluminescence imaging of transplanted neural stem cells in the brain. Contrast Media & Molecular Imaging. ,vol. 8, pp. 505- 513 ,(2013) , 10.1002/CMMI.1549
Norbert Avril, Ronald Finn, Bradley Beattie, Steven M. Larson, Farhad Daghighian, Jamal Zweit, Michelle Safer, Gene DiResta, Revathi Joshi, Takamitsu Oku, Toshio Sasajima, Jason Koutcher, Ronald Blasberg, Finn Augensen, John Humm, Juri G. Tjuvajev, Tadashi Miyagawa, Imaging herpes virus thymidine kinase gene transfer and expression by positron emission tomography. Cancer Research. ,vol. 58, pp. 4333- 4341 ,(1998)
R. A. Jefferson, T. A. Kavanagh, M. W. Bevan, GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal. ,vol. 6, pp. 3901- 3907 ,(1987) , 10.1002/J.1460-2075.1987.TB02730.X
Ronald G. Blasberg, Bernd Gansbacher, Juri G. Tjuvajev, Kyoichi Watanabe, Revathi Desai, Gunther Stockhammer, Hisao Uehara, Imaging the expression of transfected genes in vivo. Cancer Research. ,vol. 55, pp. 6126- 6132 ,(1995)
Bakhos A. Tannous, Dong-Eog Kim, Juliet L. Fernandez, Ralph Weissleder, Xandra O. Breakefield, Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo Molecular Therapy. ,vol. 11, pp. 435- 443 ,(2005) , 10.1016/J.YMTHE.2004.10.016