Serologic survey for hantavirus infection in domestic animals and coyotes from New Mexico and northeastern Arizona.

摘要: OBJECTIVE To determine whether animals had serologic evidence of infection with Sin Nombre virus (SNV). DESIGN Prospective serosurvey. SAMPLE POPULATION Serum samples were obtained from 145 cats, 85 dogs, 120 horses, and 24 cattle between April 1993 August 1994 54 coyotes December February 1995. PROCEDURE analyzed by western immunoblot assays for reaction SNV nucleocapsid antigen. Samples reactivity to proteins used probe multiple-antigen blots containing recombinant fusion derived prototypic hantaviruses. Lung tissue or blood clots in nested reverse-transcriptase polymerase chain a 320-nucleotide portion the G1 gene. RESULTS Sera 4 (2.8%) cats (3.5%) dogs trace full-length SNV-encoded proteins. All cattle, nonreactive. that IgG-antibody then tested One cat multiple cross-reactivities these hantaviruses, consistent exposure hantavirus; however, epitope mapping studies did not support this conclusion. Reverse-transcriptase lung 2 weak failed amplify any hantavirus sequence. CLINICAL IMPLICATIONS Domestic animals, particularly as well do appear have major role maintenance transmission SNV.