Two-dimensional structure of the Opc invasin from Neisseria meningitidis

摘要: A two-dimensional structural model was devised for the Opc outer membrane protein invasin which contains 10 transmembrane strands and five surface-exposed loops. One continuous epitope recognized by three monoclonal antibodies localized to tip of loop 2 synthetic peptides site-directed mutagenesis while a second, discontinuous fourth antibody loops 4 5 insertion mutagenesis. These are bactericidal inhibit adhesion invasion. Most T-cell epitopes defined Wiertz et al. (1996) were strands. Oligonucleotides encoding foreign (nabla) from Semliki Forest virus inserted into BglII restriction sites created The nabla in all predicted on cell surface live Escherichia coli bacteria exposed N-terminus or turns not. results thus confirm important predictions define permissive within can be used insert epitopes.