Specific molecular mass detection of endogenously released neuropeptides using in vivo microdialysis/mass spectrometry
作者： Mark R. Emmett , Per E. Andrén , Richard M. Caprioli
DOI: 10.1016/0165-0270(95)00070-4
关键词: Molecular mass 、 Microdialysis 、 Enkephalin 、 Mass spectrometry 、 Tandem mass spectrometry 、 Neuropeptide 、 Chemistry 、 In vivo 、 Biochemistry 、 Neurotensin
摘要: Abstract The specific molecular detection of the endogenous neuropeptides methionine ([Met]5) enkephalin and neurotensin released in vivo rat brain has been accomplished using microdialysis mass spectrometry. Microdialysis probes were implanted regions used to collect samples from extracellular fluids unanesthetized, freely moving animals. Microelectrospray/tandem spectrometry was achieve molecular-specific identification with a sensitivity amol/μl range. Measurements amounts dialysates obtained studies KCl-stimulated release showed that [Met]5-enkephalin globus pallidus/ventral pallidum region present at level approximately 4–6 fmol/10 μl dialysate hypothalamus 500 amol 10 dialysate. In this manuscript, we first data mass- quantitation individual response either intracerebrally or systemically administered compounds.
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