The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes.
作者： Chia C. Pao , Shyh-Shyan Lin , T'sang-T'ang Hsieh
DOI: 10.1016/0890-8508(90)90027-W
关键词: DNA–DNA hybridization 、 DNA 、 genomic DNA 、 Restriction enzyme 、 Hybridization probe 、 Biology 、 Molecular probe 、 Molecular biology 、 Molecular cloning 、 Microbiology 、 Gardnerella vaginalis
摘要: Cloned Gardnerella vaginalis DNA were selected from a plasmid library constructed with partially restriction endonuclease Hind III-digested genomic of G. to serve as probe in detecting vaginalis. The level detection was determined be approximately 10,000 cells by slot-blot hybridization. This will not cross-hybridize number non-Gardnerella micro-organisms commonly found female genital tract. probe-based hybridization test may become useful tool for the identification uncultured clinical specimens.
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