miR-20a regulates proliferation, differentiation and apoptosis in P19 cell model of cardiac differentiation by targeting Smoothened

摘要: ABSTRACT MicroRNA (miR)-20a, a member of the miR-17-92 cluster related to cardiac development, was obviously downregulated in myocardially differentiated P19 cells compared with normal cells. Smoothened (SMO) is Hh pathway. signaling induces differentiation cells, and SMO mediates pathway during embryonic development. Using bioinformatic prediction software Targetscan (http://www.targetscan.org/), PicTar (http://pictar.bio.nyu.edu), miRBase (http://microrna.sanger.ac.uk/), miR-20a 3′-untranslated region (3′-UTR) mRNA were predicted have complementary binding regions. Accordingly, we inferred that might act as regulator SMO, regulate proliferation, apoptosis We determined expression miR-20a, marker proteins cardiomyocytes (cTnT, GATA4 desmin) by quantitative real-time PCR (qRT-PCR) western blot assays, found had into successfully at day 10, downregulation upregulation existed Cell detection showed inhibited proliferation enhanced Moreover, verified directly targeted knockdown overexpression similar effects on cell apoptosis, which speculation inhibits enhances targeting SMO. Our results suggest may be potential target against congenital heart diseases.