The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway.
作者： Channy Park , Hong-Seob So , Sun-Ho Shin , Jin-Young Choi , In Lee
DOI: 10.1002/PTR.2028
关键词: Apoptosis 、 Biology 、 Cytosol 、 Fragmentation (cell biology) 、 Mitochondrion 、 Oxidative stress 、 Cell cycle 、 Cell biology 、 Biochemistry 、 Cytochrome c 、 Caspase
摘要: The water extract of Omija (Omija) has been used traditionally in the treatment ischemic damage heart and brain tissues. However, little is known about mechanism by which it rescues myocardial cells from oxidative stress. This study was designed to investigate protective mechanisms on H2O2-induced cytotoxicity H9c2 cardiomyoblast cells. Treatment with H2O2 resulted death cells, characterized apparent apoptotic features, including fragmentation nucleus an increase sub-G0/G1 fraction cell cycle. markedly suppressed characteristics induced H2O2. In addition, features mitochondrial dysfunction, changes membrane potential cytosolic release cytochrome c H2O2-treated further inhibited catalytic activation caspase-9 caspase-3 induction Fas Taken together, these data indicate that protects stress through inhibition dysfunction intrinsic caspase cascades, caspase-9. Copyright © 2006 John Wiley & Sons, Ltd.
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