Solubilization of sarcoplasmic reticulum membranes by sodium dodecylsulphate
作者： A. Prado , A. Muga , J. Castresana , F.M. Goñi , J.L.R. Arrondo
DOI: 10.1016/0014-5793(90)81185-Q
关键词: Membrane 、 Vesicle 、 Endoplasmic reticulum 、 Fourier transform infrared spectroscopy 、 Biological membrane 、 Chromatography 、 Pulmonary surfactant 、 Sodium 、 Protein structure 、 Chemistry
摘要: Abstract In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: mole ratios. Effects on Ca 2+ -ATPase activity, solubilization, and conformation independently monitored, correlations among the various parameters observed. The thermal denaturation proteins in presence dodecylsulphate has also characterized spectroscopically.
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