Differential Changes in Expression of Stress- and Metabolic-Related Neuropeptides in the Rat Hypothalamus during Morphine Dependence and Withdrawal

摘要: Chronic morphine treatment and naloxone precipitated withdrawal activates stress-related brain circuit results in significant changes food intake, body weight gain energy metabolism. The present study aimed to reveal hypothalamic mechanisms underlying these effects. Adult male rats were made dependent on by subcutaneous implantation of constant release drug pellets. Pair feeding revealed significantly smaller loss treated compared placebo implanted animals whose consumption was limited that eaten pairs. These suggest reduced expenditure morphine-treated animals. exposure or pair did not affect expression selected stress- metabolic related neuropeptides - corticotropin-releasing hormone (CRH), urocortin 2 (UCN2) proopiomelanocortin (POMC) fed Naloxone resulted a dramatic starting as early 15–30 min after injection increased adrenocorticotrophic hormone, prolactin corticosterone plasma levels rats. Using real-time quantitative PCR monitor the time course relative neuropeptide mRNAs hypothalamus we found elevated CRH UCN2 mRNA dramatically POMC expression. Neuropeptide Y (NPY) arginine vasopressin (AVP) transiently during opiate withdrawal. data highlight differentially affects metabolic-related rat hypothalamus, while remain unchanged either chronically with their pair-fed controls.