Role of Rac1 and Oxygen Radicals in Collagenase-1 Expression Induced by Cell Shape Change
作者： Farrah Kheradmand , Erica Werner , Patrice Tremble , Marc Symons , Zena Werb
DOI: 10.1126/SCIENCE.280.5365.898
关键词: Cell biology 、 Morphogenesis 、 Gene expression 、 Regulation of gene expression 、 Actin cytoskeleton 、 Integrin 、 Transcriptional regulation 、 Extracellular matrix 、 Biology 、 RAC1 、 Biochemistry 、 Multidisciplinary
摘要: Integrin-mediated reorganization of cell shape leads to an altered cellular phenotype. Disruption the actin cytoskeleton, initiated by binding soluble antibody alpha5beta1 integrin, led increased expression collagenase-1 gene in rabbit synovial fibroblasts. Activation guanosine triphosphate-binding protein Rac1, which was downstream necessary for this process, and activated Rac1 sufficient increase collagenase-1. activation generated reactive oxygen species that were essential nuclear factor kappa B-dependent transcriptional regulation interleukin-1alpha, which, autocrine manner, induced expression. Remodeling extracellular matrix consequent alterations integrin-mediated adhesion cytoarchitecture are central development, wound healing, inflammation, malignant disease. The resulting may lead morphogenesis, migration, invasion.
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