In vitro process for producing multiple nucleic acid copies

摘要: This invention provides inter alia an in vitro process for producing multiple specific nucleic acid copies which the are produced under isostatic conditions, e.g., temperature, buffer and ionic strength, independently of any requirement introducing intermediate structure copies. In other aspects, processes products substantially free primer-coded sequences, such sequences having been or all removed from product to regenerate a primer binding site, thereby allowing new priming events occur be produced. further promoter-independent non-naturally occurring construct that produces copy without using relying on gene may coded by construct. Another aspect this concerns protein-nucleic form conjugate linked variously, covalent linkage, complementary base-pairing, proteins, ligand receptor binding. Further disclosed is vivo introduced into cell. A still relates comprising host promoter, second promoter DNA sequence uniquely located The transcribes coding different RNA polymerase after translation capable recognizing its cognate transcribing interest with oriented it does not promote transcription different. polymerase.