In vitro, isothermal nucleic acid amplification

摘要: An isothermal method for amplifying DNA of interest having a defined 3' end and contained in target sequence is disclosed. The comprises combining, under appropriate conditions: the sequence, treated, if necessary, to render available hybridization with complementary nucleic acid sequences; promoter primer; reverse at least one RNA-dependent polymerase; DNA-dependent RNA an agent RNase H activity; nucleoside triphosphates. In addition, several embodiments amplification process are For example, restriction enzyme which recognizes specifically cleaves either single-stranded or double-stranded selected site also combined DNA, cleave site, produce end.