Method for assaying nucleic acid
作者： Juichi Saitoh , Akihiro Yokoyama , Takahiko Ishiguro
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摘要: A method for assaying a target nucleic acid, comprising: providing an RNA amplification system comprising producing double-stranded DNA using, as template, containing specific nucleotide sequence in sample, said having promoter and being capable of transcribing the or complementary to sequence, transcription product presence polymerase, using probe labeled with intercalating fluorochrome product; measuring fluorescence intensity time; calculating time when satisfies prescribed criterion based on measured change determining concentration acid sample calculated time.
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