Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater.

摘要: We have developed a homogeneous quantitative assay of DNA/RNA by performing PCR in the presence an oxazole yellow derivative, fluorescent DNA intercalative dye, and monitoring fluorescence intensity reaction mixture during cycles. demonstrated applicability this use it to quantify hepatitis C virus (HCV) RNA serum samples from patients with chronic C. This gave efficient reproducible results clinically useful dynamic range below 10(-6) copies HCV for interferon therapy.