Readout Strategies for Biomolecular Analyses
作者： Jenny Göransson
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摘要: In this letter, we demonstrate a new principle for diagnostics based on DNA sequence detection using single-stranded oligonucleotide tagged magnetic nanobeads. The target is recognized and volume-amplified to large coils by circularization of linear padlock probes through probe hybridization ligation, followed rolling circle amplification (RCA). Upon the nanobeads in RCA coils, complex magnetization spectrum beads changes dramatically, induced attached molecules. We show that can be used concentration determination down pM range, thus creating opportunity nonfluorescence-based cost-efficient high-sensitivity tool. also bead incorporation diffusion-controlled consequently may accelerated incubating sample at higher temperatures.
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