A strategy for rapid and efficient DNA sequencing by mass spectrometry
作者： Hubert Köster , Kai Tang , Dong-Jing Fu , Andreas Braun , Dirk van den Boom
DOI: 10.1038/NBT0996-1123
关键词:
摘要: Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, ladders generated on an immobilized synthetic template were resolved up to the 63-mer including primer. Detection sensivity and resolution sufficient for sequence analysis in given range. This approach is particularly suitable comparative (diagnostic) sequencing. A second method that has potential high throughput de novo also presented; it uses duplex probes five-base single-stranded overhangs capture unknown serving as primers The power demonstrated not only its very speed, but ability identify sequences readable using gel electrophoresis.
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