Method of assay of nucleic acid sequences
作者： Takahiko Ishiguro , Juichi Saitoh
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摘要: A method of assay a specific nucleic acid anticipated in sample, which comprises: DNA producing step involves production double-stranded having promoter sequence for an RNA polymerase and the (the sequence) downstream from by using sample as template, measuring single-stranded measurement RNA, wherein is initiated adding at least polymerase, ribonucleoside triphosphates probe labeled with fluorescent intercalative dye complementary to reaction solution after step, fluorescence intensity solution, carried out constant temperature, does not involve denaturing annealing procedure hybridization or separation has hybridized produced.
google.co.uk 参考文章(7)
T. Ishiguro, J. Saitoh, H. Yawata, H. Yamagishi, S. Iwasaki, Y. Mitoma, Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater. Analytical Biochemistry. ,vol. 229, pp. 207- 213 ,(1995) , 10.1006/ABIO.1995.1404
T. Ishiguro, J. Saitoh, H. Yawata, M. Otsuka, T. Inoue, Y. Sugiura, Fluorescence Detection of Specific Sequence of Nucleic Acids by Oxazole Yellow-Linked Oligonucleotides. Homogeneous Quantitative Monitoring of In Vitro Transcription Nucleic Acids Research. ,vol. 24, pp. 4992- 4997 ,(1996) , 10.1093/NAR/24.24.4992
Daniel Louis Kacian, Diane Lisa Mcallister, Sherrol Hoffa Mcdonough, Nanibhushan Dattagupta, Nucleic acid sequence amplification method, composition and kit ,(1993)
John Charles Boothroyd, Philippe Jacques Pouletty, Lawrence James Burg, Selective amplification of target polynucleotide sequences ,(1988)
Hideo Yawata, Takahiko Ishiguro, Teruhiko Inoue, Masami Otsuka, Yukio Sugiura, Method of detecting specific nucleic acid sequences ,(1995)
Thomas Raymond Gingeras, Ulrich Merten, Deborah Yantis Kwoh, Transcription-based nucleic acid amplification/detection systems ,(1988)
Yasutami Mitoma, Method for detecting or quantifying target nucleic acid ,(1991)