Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

摘要: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in vessel. The number of amplicons their to one another chosen span the required linear dynamic range reaction accommodate limitations measuring used determine amount generated. Unequal amounts distinguishable generated by providing or more components (e.g., oligomers, natural unnatural NTP an mix, like). target nucleic acid present test sample is determined detection from having within detection.