Monitoring oligonucleotide binding processes using chemiluminescence quenching
作者： Andrew James Rutter , Keith Smith , Zhaoqiang Li , Ian Weeks
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摘要: Oligonucleotide building processes are monitored by means of an oligonucleotide probe which in one embodiment is labelled at end with a chemiluminescent label and the other quencher molecule. The conformation changes according to whether hybridises substantially complementary nucleic acid sequence. In non-hybridised state sufficiently close proximity that emission attenuated, but hybridised separation such there little or no attenuation. Particular probes emitter/quencher pairs disclosed.
google.co.uk 参考文章(28)
Werner Roll, Friedrich W. Hohmann, Method of preventing metal dusting corrosion of ducts which conduct hot gas mixtures containing carbon monoxide ,(1996)
Edward T. Maggio, Kit for carrying out chemically induced fluorescence immunoassay ,(1979)
Larry Edward Morrison, Competitive homogeneous assay ,(1987)
Christopher M. Belisle, Michael J. Powell, Mark M. Richter, Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers ,(1998)
Sanjay Tyagi, Fred Russell Kramer, Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes ,(1997)
Paul M. Lizardi, Sanjay Tyagi, Fred R. Kramer, Hybridization probes for nucleic acid detection, universal stems, methods and kits ,(1994)
Mark J. Fiandaca, Brian D. Gildea, Jens J. Hyldig-Nielsen, James M. Coull, Methods, kits and compositions pertaining to linear beacons ,(1998)
Anand Natrajan, David J. Sharpe, Qingping Jiang, Wen-Jee Wong, Say-Jong Law, Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes ,(2004)
James G. Nadeau, J. Bruce Pitner, Glenn P. Vonk, Detection of nucleic acids using g-quartets ,(1997)
John T. Unger, Michael J. Lee, Eddie Carroll, Eve H. Barlow, Joseph E. Connolly, Richard A. Martinelli, Non-separation specific binding chemiluminescent assay ,(1996)