Use of RNA polymerase to improve nucleic acid amplification process

摘要: This invention relates to the use of a eukaryotic or prokaryotic DNA-directed RNA polymerase class that synthesizes cellular RNA, in process for amplification specific nucleic acid sequence its complement. It also new amplifying sequence. The includes one more reactions which may take place single reaction vessel. In instance, first reaction, providing uses DNA template synthesize an template, and, second and number other reagents such template. Thereafter cycle ensues third multiple copies is kit containing this invention.