Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.
作者： S Singer , R Ferone , L Walton , L Elwell
DOI: 10.1128/JB.164.1.470-472.1985
关键词: Methionine 、 Enterobacteriaceae 、 Biochemistry 、 Enzyme 、 Plasmid 、 Auxotrophy 、 Biology 、 Dihydrofolate reductase 、 Escherichia coli 、 Strain (chemistry)
摘要: A strain of Escherichia coli was isolated in which dihydrofolate reductase not detected by an enzyme assay or competition for antibody. This requires methionine, glycine, a purine, and thymidine growth addition to the auxotrophic requirements parent strain. It found be useful as recipient plasmids harboring genes.
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