Preparative expression and purification of a nacreous protein N16 and testing its effect on osteoporosis rat model
作者： Wei-Wei Su , Pang-Chui Shaw , Pei-Bo Li , Zhen-Yan Xu , Yu-Ling Liu
DOI: 10.1016/J.IJBIOMAC.2018.01.054
关键词: Chemistry 、 Inclusion bodies 、 Bone mineral 、 Osteoblast 、 Bone resorption 、 Biochemistry 、 Size-exclusion chromatography 、 In vivo 、 Biomineralization 、 Osteoclast
摘要: Abstract N16, a nacreous protein isolated from Pinctada martensii, is related to layer formation. Our previous study indicated that N16 showed dual regulatory effects by inducing osteoblast biomineralization as well inhibiting osteoclast In order obtain large quantity of for animal experiment and clinical trial, fermentation preparative purification method was established. The cDNA cloned BL21(DE3)plysE-pET32a vector grown in 20 L fermenter. medium, temperature, pH dissolved oxygen (DO) were optimized. expressed inclusion bodies. It denatured refolded 8 M urea buffer purified 97% purity passing through gel filtration column. glucocorticoid induced osteoporosis (GIO) rat model used investigate the anti-osteoporosis activity vivo. Results decrease bone mineral density (BMD) ultimate load significantly relieved after treatment. displayed promoting osteogenesis resorption work will contribute further studies on
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