Curcumin alleviates glucocorticoid-induced osteoporosis by protecting osteoblasts from apoptosis in vivo and in vitro.

摘要: Curcumin, an active component of the rhizomes Curcumin longa L., possesses broad anti-inflammation and anti-cancer properties. was previously reported to be capable protecting ovariectomized rats against osteoporosis. However, effect curcumin on glucocorticoid-induced osteoporosis (GIO) is not yet clear. The present study investigated effects dexamethasone (Dex)-induced in vivo Dex-induced osteoblast apoptosis vitro. GIO rat model induced by subcutaneous injection Dex for 60 days verified successful as evidenced significantly decreased bone mineral density (BMD) determined using dual X-ray absorptiometry. Subsequently, administration (100 mg/kg) obviously increased BMD bone-alkaline phosphatase, carboxy-terminal collagen cross links, enhanced mechanical strength, improved trabecular microstructure, thereby alleviating Mechanically, remarkably reversed femoral vivo. In cultured primary osteoblasts, pretreatment with concentration-dependently number apoptotic osteoblasts down-regulating ratio Bax/Bcl-2 well levels cleaved caspase-3 poly ADP-ribose polymerase (PARP). Moreover, activated extracellular signal regulated kinase (ERK) signalling up-regulating expression level p-ERK1/2. Taken together, our demonstrated that could ameliorate from apoptosis, which possibly related activation ERK pathway. results suggest may a promising drug prevention treatment GIO.