Curcumin Attenuation of Wear Particle-Induced Osteolysis via RANKL Signaling Pathway Suppression in Mouse Calvarial Model.

摘要: Wear particle-induced chronic inflammation and osteoclastogenesis are two critical factors in the osteolytic process. Curcumin (CUR) is an active compound of medicinal herb Curcuma longa has anti-inflammatory antiosteoclastogenic properties. Our study tested hypothesis that CUR might attenuate polymethylmethacrylate- (PMMA-) induced inflammatory osteolysis using mouse calvaria model vivo vitro. The mice were divided into four groups: phosphate-buffered saline group, CUR, PMMA, PMMA + CUR groups. Three days before PMMA particle implantation, intraperitoneally injected with (25 mg/kg/day). Ten after operation, was harvested for microcomputed tomography, histomorphometry, molecular biology analysis. As expected, markedly reduced secretion tumor necrosis factor-α, interleukin- (IL-) 1β, IL-6 calvarial organ culture. Moreover, suppressed decreased bone resorption compared PMMA-stimulated calvaria. Furthermore, downregulated osteoclast-specific gene expression reversed receptor activator nuclear factor kappa-B ligand (RANKL)/osteoprotegerin messenger RNA protein ratio particle-stimulated mice. These results suggest attenuated by suppressing RANKL signaling pathway murine calvarium, which could be a candidate to prevent treat AL.