In Vitro Regeneration of Curcuma caesia Plantlets from Basal Part and via Somatic Embryogenesis
作者： Ab Rahman Zuraida , Kamaruddin Fatin Liyana Izzati , Othman Ayu Nazreena , Che Mohd Zain Che Radziah , Sheikh Ghadzi Siti Nur Asyikin
关键词: Growth medium 、 Callus 、 Murashige and Skoog medium 、 Agar 、 Explant culture 、 Botany 、 Sprouting 、 Somatic embryogenesis 、 Biology 、 Aminopurine
摘要: Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds tubers. Segments the plantlets the junction between root and basal portion stem were subsequently used as explants to investigate factors affecting callus induction plant regeneration via somatic embryogenesis. The placed on Woody Plant Medium (WPM) together with different concentrations 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in presence light. growth medium supplemented with 5 mg/L BAP 2 2,4-D promoted callus induction after 70 days culture. Sub-culturing same medium enhanced production of friable callus. Culture media containing higher agar promoted the development green somatic embryos Respond somatic embryogenesis was most successful MS 6.0 g/L supplemented with 0.2 whereby developed into green somatic an efficiency 53%. This culture also produced the largest number plantlets.
sci-hub.se 参考文章(28)
A. V. Roberts, K. Yokoya, S. Walker, J. Mottley, Somatic embryogenesis in Rosa spp. Forestry sciences.. ,vol. 46, pp. 277- 289 ,(1995) , 10.1007/978-94-011-0491-3_15
L. Martinelli, I. Gribaudo, Somatic embryogenesis in grapevine Springer Netherlands. pp. 327- 351 ,(2001) , 10.1007/978-94-017-2308-4_13
J. R. Kikkert, M. R. Thomas, B. I. Reisch, Grapevine Genetic Engineering Springer Netherlands. pp. 393- 410 ,(2001) , 10.1007/978-94-017-2308-4_15
A. S. Mohmand, Induced variability for some agronomic and morphological characters in wheat. Pakistan Journal of Agricultural Research. ,vol. 15, pp. 100- 107 ,(1994)
Neeta D. Salvi, Leela George, Susan Eapen, Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants Plant Cell Tissue and Organ Culture. ,vol. 66, pp. 113- 119 ,(2001) , 10.1023/A:1010638209377
Magali F. Grando, Chandra I. Franklin, Robert G. Shatters Jr., Optimizing embryogenic callus production and plant regeneration from 'Tifton 9' bahiagrass seed explants for genetic manipulation Plant Cell Tissue and Organ Culture. ,vol. 71, pp. 213- 222 ,(2002) , 10.1023/A:1020303522530
Azra Quraishi, Hamid Rashid, Zubeda Chaudhry, Somatic embryogenesis in citrus. Pakistan Journal of Agricultural Research. ,vol. 15, pp. 14- 18 ,(1994)
Sanghamitra Nayak, In vitro multiplication and microrhizome induction in Curcuma aromatica Salisb. Plant Growth Regulation. ,vol. 32, pp. 41- 47 ,(2000) , 10.1023/A:1006307316393
Shyamala Bhaskaran, Roberta H. Smith, Regeneration in cereal tissue culture: a review. Crop Science. ,vol. 30, pp. 1328- 1337 ,(1990) , 10.2135/CROPSCI1990.0011183X003000060034X
S. Mohanty, M. K. Panda, E. Subudhi, S. Nayak, Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis Biologia Plantarum. ,vol. 52, pp. 783- 786 ,(2008) , 10.1007/S10535-008-0153-X