Optimizing embryogenic callus production and plant regeneration from 'Tifton 9' bahiagrass seed explants for genetic manipulation

摘要: Bahiagrass (Paspalum notatum Flugge) is a warm season forage grass widely cultivated in southeastern U.S. and South America. The cultivar Tifton 9 has several desirable characteristics such as high yield, more vigor at the seedling stage, etc.; but its quality very low. As an initial step for future genetic manipulations to improve characteristics, we have optimized vitro culture conditions plant regeneration. In this report, describe efficient method embryogenic callus induction regeneration from bahiagrass (cv. 9) seed explants, which are readily available easy manipulate, compared other explant sources reported literature. Murashige Skoog (MS) medium containing 30 μM dicamba 5 6-benzyladenine (BA) was optimal growth. Out of 9734 seeds cultured, 65.7% germinated 21.4% produced on medium. Shoot formation best when calluses induced were transferred MS supplemented with BA 1 gibberellic acid 1640 plantlets formed per gram fresh weight tissue. When hormone-free SH medium, shoot systems well-developed root systems. resulting grew normally viable soil greenhouse. Histochemical staining GUS activity arising transient expression introduced uidA (β-glucuronidase) gene indicated that by suitable transfer via biolistic bombardment; it can serve good target tissue 9).