Natural antisense transcripts in Plasmodium falciparum isolates from patients with complicated malaria

摘要: Mechanisms regulating gene expression in malaria parasites are not well understood. Little is known about how the parasite regulates its during transition from one developmental stage to another and response various environmental conditions. Parasites a diseased host face environments which differ static, adapted vitro thus need adapt quickly effectively these conditions by establishing transcriptional states best suited for better survival. With discovery of natural antisense transcripts (NATs) this considering proposed mechanisms NATs might regulate expression, it has been speculated that be playing critical role regulation. We report here diversity parasite, using isolates taken directly patients with differing clinical symptoms caused infection. Using custom designed strand specific whole genome microarray, total 797 targeted against annotated loci have detected. Out these, 545 unique study. The majority were positively correlated pattern sense transcript. However, 96 genes showed change sense/antisense ratio on comparison between uncomplicated complicated disease map broad range biochemical/metabolic pathways, especially pathways pertaining central carbon metabolism stress related pathways. Our data strongly suggests large group detected unannotated transcription units models. results reveal previously unknown set prevails their differential regulation mapping functionally genes. detailed call studies deduce possible mechanism action NATs, would further help understanding vivo pathological adaptations parasites.