Interleukin-8 gene silencing on pancreatic cancer cells using biodegradable polymer nanoplexes.

摘要: Pancreatic cancer is one of the deadliest cancers throughout world with rarely efficient therapies currently available. Gene therapy on pancreatic through small interfering RNA (siRNA)-based interference (RNAi) has shown great potential and attracted much attention. However, due to fragile nature nucleic acid, application RNAi as a safe carrier faces challenges. In this contribution, self-assembly regime, which based well-defined cationic polylactides (CPLAs) tertiary amine groups, been used encapsulate protect siRNAs from fast degradation. CPLA degradable formulation that allowed us deliver targeting proangiogenic chemokine interleukin-8 (IL-8) cells for gene therapy. Stable IL-8 siRNA–CPLA nanoplexes were successfully formed by electrostatic force high transfection efficiencies two cell lines. We did not observe any cytotoxicity these CPLAs over large concentration range via viability evaluations. More importantly, silencing expression significantly attenuated proliferation cells. Our preliminary results support future development might provide an effective treatment approach towards cancer.